TGF-beta 3 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 158 476 935 182 563 1138 SD 7.43 20.5 50.5 10.56 32.1 74 CV% 4.7 4.3 5.4 5.8 5.7 6.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 158 476 935 182 563 1138 Mean (pg/mL) 158 476 935 182 563 1138 SD 7.43 20.5 50.5 10.56 32.1 74 SD 7.43 20.5 50.5 10.56 32.1 74 CV% 4.7 4.3 5.4 5.8 5.7 6.5 CV% 4.7 4.3 5.4 5.8 5.7 6.5

• Gene Name:

  Transforming growth factor, beta 3

• Gene Aliases:

  ARVD, ARVD1, FLJ16571, MGC105479, MGC118722, prepro-transforming growth factor beta-3, RNHF, TGF beta 3, TGF beta3, TGF-beta-3, TGFB 3, TGFB3, TGFB3_HUMAN, transforming growth factor beta 3, Transforming growth factor beta-3

• Gene ID:

  21809

• Accession Number:

  NP_033394

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A301-S412

• Target:

  Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse TGF-beta 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF-beta 3 has been precoated onto 96-well plates. Standards (NS0, A301-S412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TGF-beta 3 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants, serum, plasma (EDTA) and urine

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse TGF-beta 3

• NCBI Gene Symbol:

  TGFB3

• Protein Name:

  Transforming growth factor beta-3

• Gene Name URL:

  TGFB3

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009368