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CD200 ELISA Kit (Human)

CAT: 0247-OKBB00486-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00486-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 120 635 1149 130 623 1208 SD 6.12 34.29 70.09 8.06 35.51 90.6 CV% 5.1 5.4 6.1 6.2 5.7 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 120 635 1149 130 623 1208 Mean (pg/mL) 120 635 1149 130 623 1208 SD 6.12 34.29 70.09 8.06 35.51 90.6 SD 6.12 34.29 70.09 8.06 35.51 90.6 CV% 5.1 5.4 6.1 6.2 5.7 7.5 CV% 5.1 5.4 6.1 6.2 5.7 7.5
CAS Number
7732-18-5
Gene Name
CD200 molecule
Gene Aliases
Antigen identified by monoclonal antibody MRC OX-2, CD_antigen=CD200, CD200 antigen, MOX1, MOX2, MRC OX-2 antigen, My033, OX-2 membrane glycoprotein
Gene ID
4345
Accession Number
NP_001004196.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q31-G232
Target
OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD200 has been precoated onto 96-well plates. Standards (NS0, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CD200 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CD200
NCBI Gene Symbol
CD200
Protein Name
OX-2 membrane glycoprotein
Gene Name URL
CD200
Nucleotide Accession Number
NM_001004196.2

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