CD200 ELISA Kit (Human)

• Reproducibility :

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 120 635 1149 130 623 1208 SD 6.12 34.29 70.09 8.06 35.51 90.6 CV% 5.1 5.4 6.1 6.2 5.7 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 120 635 1149 130 623 1208 Mean (pg/mL) 120 635 1149 130 623 1208 SD 6.12 34.29 70.09 8.06 35.51 90.6 SD 6.12 34.29 70.09 8.06 35.51 90.6 CV% 5.1 5.4 6.1 6.2 5.7 7.5 CV% 5.1 5.4 6.1 6.2 5.7 7.5

• Gene Name :

  CD200 molecule

• Gene Aliases :

  Antigen identified by monoclonal antibody MRC OX-2, CD_antigen=CD200, CD200 antigen, MOX1, MOX2, MRC OX-2 antigen, My033, OX-2 membrane glycoprotein

• Gene ID :

  4345

• Accession Number :

  NP_001004196.2

• Reactivity :

  Human

• Immunogen :

  Expression system for standard: NS0; Immunogen sequence: Q31-G232

• Target :

  OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

• Type :

  ELISA Kit

• Applications :

  ELISA

• Assay Principle :

  Aviva's human CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD200 has been precoated onto 96-well plates. Standards (NS0, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CD200 amount of sample captured in plate.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Cell culture supernatants, serum and plasma (heparin, EDTA)

• Detection Range :

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity :

  <10 pg/ml

• Reconstitution :

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity :

  Natural and recombinant human CD200

• NCBI Gene Symbol :

  CD200

• Protein Name :

  OX-2 membrane glycoprotein

• Gene Name URL :

  CD200

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_001004196.2