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TEK/TIE2 ELISA Kit (Mouse)

CAT: 0247-OKBB00481-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00481-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 454 1225 4837 632 1586 5124 SD 30.9 77.2 285.4 46.14 103.1 312.6 CV% 6.8 6.3 5.9 7.3 6.5 6.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 454 1225 4837 632 1586 5124 Mean (pg/mL) 454 1225 4837 632 1586 5124 SD 30.9 77.2 285.4 46.14 103.1 312.6 SD 30.9 77.2 285.4 46.14 103.1 312.6 CV% 6.8 6.3 5.9 7.3 6.5 6.1 CV% 6.8 6.3 5.9 7.3 6.5 6.1
CAS Number
7732-18-5
Gene Name
TEK tyrosine kinase, endothelial
Gene Aliases
Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2, variant 1, soluble TIE2 variant 2, Tek, TEK tyrosine kinase, endothelial, TIE 2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1
Gene ID
21687
Accession Number
NP_001277478.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: A23-K744
Target
Angiopoietin-1 receptor, also called TEK or TIE2 is a protein that in humans is encoded by the TEK gene. This gene is mapped to 9p21.2. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse TEK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for TEK has been precoated onto 96-well plates. Standards (NS0, A23-K744) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TEK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TEK amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, tissue homogenates, serum and plasma (heparin, EDTA)
Detection Range
125 pg/ml - 8,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse TEK
NCBI Gene Symbol
TEK
Protein Name
Angiopoietin-1 receptor
Gene Name URL
TEK
Nucleotide Accession Number
NM_001290549.1

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