ADAM12 ELISA Kit (Human)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.5 4.2 7.4 1.6 4.1 7.2 SD 0.07 0.18 0.29 0.09 0.21 0.36 CV% 4.3 3.9 5.6 5.1 5.1 5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.5 4.2 7.4 1.6 4.1 7.2 Mean (pg/mL) 1.5 4.2 7.4 1.6 4.1 7.2 SD 0.07 0.18 0.29 0.09 0.21 0.36 SD 0.07 0.18 0.29 0.09 0.21 0.36 CV% 4.3 3.9 5.6 5.1 5.1 5 CV% 4.3 3.9 5.6 5.1 5.1 5

• Gene Name:

  ADAM metallopeptidase domain 12

• Gene Aliases:

  ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin-alpha, MLTN, MLTNA

• Gene ID:

  8038

• Accession Number:

  NP_001275903.1

• Reactivity:

  Human

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: R29-S513

• Target:

  ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's human ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been precoated onto 96-well plates. Standards (CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ADAM12 amount of sample captured in plate. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants, serum, plasma (heparin) and urine

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant human ADAM12

• NCBI Gene Symbol:

  ADAM12

• Protein Name:

  Disintegrin and metalloproteinase domain-containing protein 12

• Gene Name URL:

  ADAM12

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001288974.1