COMP ELISA Kit (Human)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.02 3.12 6.19 1.13 3.08 6.23 SD 0.052 0.131 0.254 0.07 0.166 0.324 CV% 5.1 4.2 4.1 6.2 5.4 5.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.02 3.12 6.19 1.13 3.08 6.23 Mean (pg/mL) 1.02 3.12 6.19 1.13 3.08 6.23 SD 0.052 0.131 0.254 0.07 0.166 0.324 SD 0.052 0.131 0.254 0.07 0.166 0.324 CV% 5.1 4.2 4.1 6.2 5.4 5.2 CV% 5.1 4.2 4.1 6.2 5.4 5.2

• Gene Name:

  Cartilage oligomeric matrix protein

• Gene Aliases:

  Cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple), Cartilage oligomeric matrix protein, Cartilage oligomeric matrix protein precursor, COMP, COMP_HUMAN, EDM 1, EDM1, EPD 1, EPD1, Epiphyseal dysplasia 1, Epiphyseal dysplasia 1 multiple, Epiphyseal dysplasia multiple 1, MED, MGC13181, MGC149768, PSACH, pseudoachondroplasia (epiphyseal dysplasia 1, multiple), Pseudoachondroplasia, THBS 5, THBS5, Thrombospondin 5, Thrombospondin-5, Thrombospondin5, TSP5

• Gene ID:

  1311

• Accession Number:

  NP_000086.2

• Reactivity:

  Human

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: Q21-A757

• Target:

  Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's human COMP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for COMP has been precoated onto 96-well plates. Standards (NS0, Q21-A757) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human COMP amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants, serum and plasma (heparin, EDTA)

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant human COMP

• NCBI Gene Symbol:

  COMP

• Protein Name:

  Cartilage oligomeric matrix protein

• Gene Name URL:

  COMP

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_000095.2