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COMP ELISA Kit (Human)

CAT: 0247-OKBB00457-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#: OKBB00457-96WSize: 96 Well With Removable Strips
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AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.02 3.12 6.19 1.13 3.08 6.23 SD 0.052 0.131 0.254 0.07 0.166 0.324 CV% 5.1 4.2 4.1 6.2 5.4 5.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.02 3.12 6.19 1.13 3.08 6.23 Mean (pg/mL) 1.02 3.12 6.19 1.13 3.08 6.23 SD 0.052 0.131 0.254 0.07 0.166 0.324 SD 0.052 0.131 0.254 0.07 0.166 0.324 CV% 5.1 4.2 4.1 6.2 5.4 5.2 CV% 5.1 4.2 4.1 6.2 5.4 5.2
CAS Number
7732-18-5
Gene Name
Cartilage oligomeric matrix protein
Gene Aliases
Cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple), Cartilage oligomeric matrix protein, Cartilage oligomeric matrix protein precursor, COMP, COMP_HUMAN, EDM 1, EDM1, EPD 1, EPD1, Epiphyseal dysplasia 1, Epiphyseal dysplasia 1 multiple, Epiphyseal dysplasia multiple 1, MED, MGC13181, MGC149768, PSACH, pseudoachondroplasia (epiphyseal dysplasia 1, multiple), Pseudoachondroplasia, THBS 5, THBS5, Thrombospondin 5, Thrombospondin-5, Thrombospondin5, TSP5
Gene ID
1311
Accession Number
NP_000086.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q21-A757
Target
Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human COMP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for COMP has been precoated onto 96-well plates. Standards (NS0, Q21-A757) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human COMP amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human COMP
NCBI Gene Symbol
COMP
Protein Name
Cartilage oligomeric matrix protein
Gene Name URL
COMP
Nucleotide Accession Number
NM_000095.2

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