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IGFBP2 ELISA Kit (Mouse)

CAT: 0247-OKBB00398-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00398-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1257 3204 5394 1020 3562 4975 SD 89.2 166.6 350.61 84.66 239 368.15 CV% 7.1 5.2 6.5 8.3 6.7 7.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1257 3204 5394 1020 3562 4975 Mean (pg/mL) 1257 3204 5394 1020 3562 4975 SD 89.2 166.6 350.61 84.66 239 368.15 SD 89.2 166.6 350.61 84.66 239 368.15 CV% 7.1 5.2 6.5 8.3 6.7 7.4 CV% 7.1 5.2 6.5 8.3 6.7 7.4
CAS Number
7732-18-5
Gene Name
Insulin-like growth factor binding protein 2, 36kDa
Gene Aliases
BP 2, BP2, IBP 2, IBP-2, IBP2, IBP2_HUMAN, IGF binding protein 2, IGF BP53, IGF-binding protein 2, IGF-BP53, IGFBP-2, IGFBP2, IGFBP53, Insulin Like Growth Factor Binding Protein 2, Insulin like growth factor binding protein 2 36kDa, Insulin like growth factor-binding protein 2 precursor, Insulin-like growth factor-binding protein 2
Gene ID
16008
Accession Number
NP_032368.2
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: E35-Q305
Target
Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP2 has been precoated onto 96-well plates. Standards (NS0, E35-Q305) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IGFBP2 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
125 pg/ml - 8,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse IGFBP2
NCBI Gene Symbol
IGFBP2
Protein Name
Insulin-like growth factor-binding protein 2
Gene Name URL
IGFBP2
Nucleotide Accession Number
NM_008342.3

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