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SAA ELISA Kit (Mouse)

CAT: 0247-OKBB00384-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00384-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.4 3.4 5.7 1.5 3.6 6.8 SD 0.06 0.177 0.291 0.081 0.223 0.388 CV% 4.3 5.2 5.1 5.4 6.2 5.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.4 3.4 5.7 1.5 3.6 6.8 Mean (pg/mL) 1.4 3.4 5.7 1.5 3.6 6.8 SD 0.06 0.177 0.291 0.081 0.223 0.388 SD 0.06 0.177 0.291 0.081 0.223 0.388 CV% 4.3 5.2 5.1 5.4 6.2 5.7 CV% 4.3 5.2 5.1 5.4 6.2 5.7
CAS Number
7732-18-5
Gene Name
Serum amyloid A1
Gene Aliases
Amyloid fibril protein AA, Amyloid protein A, MGC111216, OTTMUSP00000045357, PIG4, SAA, SAA1, Serum amyloid A 1, Serum amyloid A 1 protein, serum amyloid A protein, Serum amyloid A protein precursor, Serum amyloid A1 isoform 1, Serum amyloid A1 isoform 2, TP53I4, Tumor protein p53 inducible protein 4
Gene ID
111345
Accession Number
NP_033143.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: G20-Y122
Target
Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse SAA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for SAA has been precoated onto 96-well plates. Standards (E.coli, G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse SAA amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Detection Range
1.56 ng/ml - 100 ng/ml
Sensitivity
<150 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse SAA
NCBI Gene Symbol
SAA1
Protein Name
Serum amyloid A-1 protein
Gene Name URL
SAA1
Nucleotide Accession Number
NM_009117.3