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CEA ELISA Kit (Human)

CAT: 0247-OKBB00360-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00360-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (ng/mL) 2.36 5.59 10.4 2.48 5.56 10.7 St. Dev. 0.113 0.235 0.53 0.171 0.395 0.556 %CV 4.78 4.20 5.09 6.89 7.10 5.19 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (ng/mL) 2.36 5.59 10.4 2.48 5.56 10.7 Mean (ng/mL) 2.36 5.59 10.4 2.48 5.56 10.7 St. Dev. 0.113 0.235 0.53 0.171 0.395 0.556 St. Dev. 0.113 0.235 0.53 0.171 0.395 0.556 %CV 4.78 4.20 5.09 6.89 7.10 5.19 %CV 4.78 4.20 5.09 6.89 7.10 5.19
CAS Number
7732-18-5
Gene Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Gene Aliases
Carcinoembryonic antigen, Carcinoembryonic antigen related cell adhesion molecule 5, CD66e, CD66e antigen, CEA, CEACAM 5, CEACAM5, DKFZp781M2392, Meconium antigen 100
Gene ID
111518
Accession Number
NP_001278413.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: K35-A685
Target
Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CEA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEA has been precoated onto 96-well plates. Standards (NS0, K35-A685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CEA amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CEA
NCBI Gene Symbol
CEACAM5
Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Gene Name URL
CEACAM5
Nucleotide Accession Number
NM_001291484.2

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