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KIM1 ELISA Kit (Human)

CAT: 0247-OKBB00355-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00355-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 253 746 1220 317 882 1458 St. Dev. 11.64 38.8 74.42 20.3 59.1 115.2 %CV 4.60 5.20 6.1 6.40 6.70 7.90 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 253 746 1220 317 882 1458 Mean (pg/mL) 253 746 1220 317 882 1458 St. Dev. 11.64 38.8 74.42 20.3 59.1 115.2 St. Dev. 11.64 38.8 74.42 20.3 59.1 115.2 %CV 4.60 5.20 6.1 6.40 6.70 7.90 %CV 4.60 5.20 6.1 6.40 6.70 7.90
CAS Number
7732-18-5
Gene Name
Hepatitis A virus cellular receptor 1
Gene Aliases
HAVCR, HAVcr-1, Havcr1, HAVR1_HUMAN, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM-1, T cell immunoglobin domain and mucin domain protein 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM-1, TIM1, TIMD-1, TIMD1
Gene ID
26762
Accession Number
NP_001166864.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: S21-T288
Target
KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, the HAVCR1 gene was mapped to 5q33.2.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for KIM1 has been precoated onto 96-well plates. Standards (NS0, S21-T288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human KIM1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum, plasma (heparin, EDTA) and urine
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<2 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human KIM1
NCBI Gene Symbol
HAVCR1
Protein Name
Hepatitis A virus cellular receptor 1
Gene Name URL
HAVCR1
Nucleotide Accession Number
NM_001173393.2

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