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CXCL16 ELISA Kit (Mouse)

CAT: 0247-OKBB00317-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00317-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 125 357 556 130 349 618 St. Dev. 6 21.8 28.9 7.9 25.8 38.9 %CV 4.8 6.10 5.19 6.07 7.39 6.29 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 125 357 556 130 349 618 Mean (pg/mL) 125 357 556 130 349 618 St. Dev. 6 21.8 28.9 7.9 25.8 38.9 St. Dev. 6 21.8 28.9 7.9 25.8 38.9 %CV 4.8 6.10 5.19 6.07 7.39 6.29 %CV 4.8 6.10 5.19 6.07 7.39 6.29
CAS Number
7732-18-5
Gene Name
Chemokine (C-X-C motif) ligand 16
Gene Aliases
0910001K24Rik, AV290116, BB024863, C X C motif chemokine 16, Chemokine (C X C motif) ligand 16, CXC chemokine ligand 16, CXCLG16, DN 491F10.6, Scavenger receptor for phosphatidylserine and oxidized low absorbance lipoprotein, SCYB16, Small inducible cytokine B, Small inducible cytokine B16, Small inducible cytokine B16 precursor, SR PSOX, SR PSOX/CXCL16 scavenger receptor for phosphatidylserine and oxidized low absorbance lipoprotein, SRPSOX, Transmembrane chemokine CXCL16, Zinc finger, MYND-type containing 15, Zmynd15
Gene ID
66102
Accession Number
NP_075647.3
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N27-P114
Target
Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2.1 These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine.2 CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen.1 Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells.1 CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo.1, 3 Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13.4 The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse CXCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL16 has been precoated onto 96-well plates. Standards (E.coli, N27-P114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CXCL16 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse CXCL16
NCBI Gene Symbol
CXCL16
Protein Name
C-X-C motif chemokine 16
Gene Name URL
CXCL16
Nucleotide Accession Number
NM_023158.6

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