Products for Research Use Only
RegisterLogin

VEGF ELISA Kit (Mouse)

CAT: 0247-OKBB00273-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00273-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 126 341 580 212 475 624 St. Dev. 4.41 13.64 28.42 10.6 30.4 46.8 %CV 3.5 4 4.9 5 6.4 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 126 341 580 212 475 624 Mean (pg/mL) 126 341 580 212 475 624 St. Dev. 4.41 13.64 28.42 10.6 30.4 46.8 St. Dev. 4.41 13.64 28.42 10.6 30.4 46.8 %CV 3.5 4 4.9 5 6.4 7.5 %CV 3.5 4 4.9 5 6.4 7.5
CAS Number
7732-18-5
Gene Name
Vascular endothelial growth factor A
Gene Aliases
MGC70609, MVCD1, Vascular endothelial growth factor, Vascular endothelial growth factor A, Vascular permeability factor, VEGF A, VEGFA, VPF
Gene ID
22339
Accession Number
NP_001020421.2
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: A27-R190
Target
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias.VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGF has been precoated onto 96-well plates. Standards (NS0, A27-R190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse VEGF amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<2 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF
NCBI Gene Symbol
VEGFA
Protein Name
Vascular endothelial growth factor A
Gene Name URL
VEGFA
Nucleotide Accession Number
NM_001025250.3

Related Products

CatalogName
OKBB00272-96WVEGF ELISA Kit (Rat)
OKBB00271-96WVEGF ELISA Kit (Human)
OKCD06067-96WVEGF ELISA Kit (Rabbit)
OKRC01330-96WOKRC01330-96W - VEGF R1 ELISA Kit (Mouse)
OKRC01328-96WOKRC01328-96W - VEGF-B ELISA Kit (Mouse)
OKBB00283-96WEG-VEGF ELISA Kit (Human)
OKBB00271VEGF ELISA Kit (Human) (OKBB00271)
OKCD06067VEGF ELISA Kit (Rabbit) (OKCD06067)
OKBB00272VEGF ELISA Kit (Rat) (OKBB00272)
OKRC01378-96WOKRC01378-96W - VEGF-A ELISA Kit (Pig)

Alternative Products

CatalogName
EA-2401Mouse VEGF ELISA
DIY0746M-01Mouse VEGF-A ELISA
YP-W21898-01Mouse VEGF-D (VEGF-D) ELISA Kit
BEK1227Mouse VEGF ELISA Kit
MBS691463-01Mouse VEGF-A ELISA (total)
I2143VEGF) Mouse ELISA Kit
JP27102VEGF mouse ELISA Kit
KET7016-01ELISA kit for Mouse VEGF
DY493Mouse VEGF DuoSet ELISA
E072354Mouse VEGF-A ELISA Kit

Popular Products