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TRAIL ELISA Kit (Human)

CAT: 0247-OKBB00266-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00266-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 102 368 637 167 326 624 St. Dev. 4.59 12.88 39.5 13.36 24.45 48.67 %CV 4.5 3.5 6.20 8 7.5 7.79 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 102 368 637 167 326 624 Mean (pg/mL) 102 368 637 167 326 624 St. Dev. 4.59 12.88 39.5 13.36 24.45 48.67 St. Dev. 4.59 12.88 39.5 13.36 24.45 48.67 %CV 4.5 3.5 6.20 8 7.5 7.79 %CV 4.5 3.5 6.20 8 7.5 7.79
CAS Number
7732-18-5
Gene Name
Tumor necrosis factor (ligand) superfamily, member 10
Gene Aliases
Apo 2 ligand, APO2L, CD253, Protein TRAIL, TL2, TNF related apoptosis inducing ligand, Tumor necrosis factor ligand superfamily member 10
Gene ID
8743
Accession Number
NP_001177871.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: T95-G281
Target
In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253) .Tumor necrosis factor (TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII) . The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2) . The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G281.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human TRAIL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRAIL has been precoated onto 96-well plates. Standards (NS0, T95-G281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRAIL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TRAIL amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum, plasma (heparin, EDTA) and saliva.
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Notes
Formerly GWB-ZZD166_x005F
Specificity
Natural and recombinant human TRAIL
NCBI Gene Symbol
TNFSF10
Protein Name
Tumor necrosis factor ligand superfamily member 10
Gene Name URL
TNFSF10
Nucleotide Accession Number
NM_001190942.1

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