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TGFI ELISA Kit (Human)

CAT: 0247-OKBB00252-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00252-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 118 225 516 126 238 509 St. Dev. 5.43 12.83 16.5 8.95 14.28 27 %CV 4.60 5.70 3.19 7.10 6 5.30 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 118 225 516 126 238 509 Mean (pg/mL) 118 225 516 126 238 509 St. Dev. 5.43 12.83 16.5 8.95 14.28 27 St. Dev. 5.43 12.83 16.5 8.95 14.28 27 %CV 4.60 5.70 3.19 7.10 6 5.30 %CV 4.60 5.70 3.19 7.10 6 5.30
CAS Number
7732-18-5
Gene Name
Transforming growth factor, alpha
Gene Aliases
EGF like TGF, ETGF, OTTHUMP00000203300, OTTHUMP00000203301, OTTHUMP00000203345, OTTHUMP00000203352, Protransforming growth factor alpha, RATTGFAA, TFGA, TGF A, TGF type 1, TGFA, Transforming growth factor alpha, Transforming growth factor alpha precursor, Wa1, Waved 1
Gene ID
7039
Accession Number
NP_001093161.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V40-A89
Target
Transforming growth factor alpha (TGF-α) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGFα stimulates neural cell proliferation in the adult injured brain.1 Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma.2 Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.3
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human TGF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli, V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TGF alpha amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum, plasma (heparin, EDTA) and human milk
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Homology
Cross-reactivates with TGFβ2, TGFβ3, TGFβ5 <1%.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human TGF alpha
NCBI Gene Symbol
TGFA
Protein Name
Protransforming growth factor alpha
Gene Name URL
TGFA
Nucleotide Accession Number
NM_001099691.2

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