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PLGF ELISA Kit (Human)

CAT: 0247-OKBB00242-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00242-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 52 146 526 45 165 583 St. Dev. 3.28 8.47 39.5 3.24 10.9 49 %CV 6.30 5.80 7.50 7.2 6.60 8.40 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 52 146 526 45 165 583 Mean (pg/mL) 52 146 526 45 165 583 St. Dev. 3.28 8.47 39.5 3.24 10.9 49 St. Dev. 3.28 8.47 39.5 3.24 10.9 49 %CV 6.30 5.80 7.50 7.2 6.60 8.40 %CV 6.30 5.80 7.50 7.2 6.60 8.40
CAS Number
7732-18-5
Gene Name
Placental growth factor
Gene Aliases
AI854365, D12S1900, MGC93298, Pgf, PGFL, PIGF, Placenta growth factor, Placental growth factor, Placental growth factor vascular endothelial growth factor related protein, PlGF, PlGF 2, PLGF_HUMAN, PlGF2, SHGC 10760, Vascular endothelial growth factor related protein
Gene ID
5228
Accession Number
NP_001193941.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A21-R149
Target
The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human PLGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLGF has been precoated onto 96-well plates. Standards (E.coli, A21-R149) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human PLGF amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human PLGF
NCBI Gene Symbol
PGF
Protein Name
Placenta growth factor
Gene Name URL
PGF
Nucleotide Accession Number
NM_001207012.1

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