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PDGF-AB ELISA Kit (Mouse)

CAT: 0247-OKBB00241-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00241-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 262 524 924 280 649 1107 St. Dev. 12.3 27.8 57.3 15.4 41.54 80.8 %CV 4.69 5.30 6.20 5.5 6.40 7.29 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 262 524 924 280 649 1107 Mean (pg/mL) 262 524 924 280 649 1107 St. Dev. 12.3 27.8 57.3 15.4 41.54 80.8 St. Dev. 12.3 27.8 57.3 15.4 41.54 80.8 %CV 4.69 5.30 6.20 5.5 6.40 7.29 %CV 4.69 5.30 6.20 5.5 6.40 7.29
CAS Number
7732-18-5
Gene Name
Platelet-derived growth factor beta polypeptide, platelet-derived growth factor alpha polypeptide
Gene Aliases
PDGF A chain, PDGF B chain, PDGF1, PDGF2, PDGFA, PDGFB, Platelet derived growth factor AB heterodimer
Gene ID
18591; 18590
Accession Number
NP_035187.2
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: (S87-T211) + (S82-T190)
Target
The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV) . It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse PDGF-AB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PDGF-AB has been precoated onto 96-well plates. Standards (E.coli, (S87-T211) + (S82-T190) ) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-AB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse PDGF-AB amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<3 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse PDGF-AB
NCBI Gene Symbol
PDGFB, PDGFA
Protein Name
Platelet-derived growth factor subunit B, Platelet-derived growth factor subunit A
Gene Name URL
PDGFB
Nucleotide Accession Number
NM_011057.3

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