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MMP-2 ELISA Kit (Mouse)

CAT: 0247-OKBB00221-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00221-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 779 3542 15328 844 4217 13668 St. Dev. 41.28 170.01 950.33 54.01 231.93 997.76 %CV 5.29 4.79 6.19 6.39 5.49 7.29 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 779 3542 15328 844 4217 13668 Mean (pg/mL) 779 3542 15328 844 4217 13668 St. Dev. 41.28 170.01 950.33 54.01 231.93 997.76 St. Dev. 41.28 170.01 950.33 54.01 231.93 997.76 %CV 5.29 4.79 6.19 6.39 5.49 7.29 %CV 5.29 4.79 6.19 6.39 5.49 7.29
CAS Number
7732-18-5
Gene Name
Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Gene Aliases
72 kDa gelatinase, 72 kDa type IV collagenase, CLG 4, CLG 4A, CLG4, CLG4A, Collagenase type IV A, EC 3.4.24.24, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil, Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix metalloproteinase 2, Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase), Matrix metalloproteinase II, Matrix metalloproteinase-2, MMP 2, MMP II, MMP-2, MMP2, MMP2_HUMAN, MONA, Neutrophil gelatinase, PEX, TBE 1, TBE-1
Gene ID
17390
Accession Number
NP_032636.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: A30-C662
Target
Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2) . It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch) . The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant mouse MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-2 has been precoated onto 96-well plates. Standards (NS0, A30-C662) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse MMP-2 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin)
Detection Range
625 pg/ml - 40,000 pg/ml
Sensitivity
<10 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse total MMP-2
NCBI Gene Symbol
MMP2
Protein Name
72 kDa type IV collagenase
Gene Name URL
MMP2
Nucleotide Accession Number
NM_008610.3

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