M-CSF ELISA Kit (Mouse)

CAT: 0247-OKBB00212-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00212-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 92 316 628 127 439 685 St. Dev. 5.06 19.28 40.2 7.493 27.66 51.4 %CV 5.5 6.10 6.40 5.9 6.30 7.50 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 92 316 628 127 439 685 Mean (pg/mL) 92 316 628 127 439 685 St. Dev. 5.06 19.28 40.2 7.493 27.66 51.4 St. Dev. 5.06 19.28 40.2 7.493 27.66 51.4 %CV 5.5 6.10 6.40 5.9 6.30 7.50 %CV 5.5 6.10 6.40 5.9 6.30 7.50
CAS Number
7732-18-5
Gene Name
Colony stimulating factor 1
Gene Aliases
Colony stimulating factor 1, CSF-1, CSF1, Lanimostim, MCSF, P09603, CSF 1, M CSF, M-CSF
Gene ID
12977
Accession Number
NP_001107001.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: K33-E262
Target
SF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for M-CSF has been precoated onto 96-well plates. Standards (E.coli, K33-E262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse M-CSF amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, tissue homogenates, serum and plasma (EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Notes
Formerly GWB-ZZD125
Specificity
Natural and recombinant mouse M-CSF
NCBI Gene Symbol
CSF1
Protein Name
Macrophage colony-stimulating factor 1
Gene Name URL
CSF1
Nucleotide Accession Number
NM_001113529.1

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