Products for Research Use Only
RegisterLogin

IL-4 ELISA Kit (Rat)

CAT: 0247-OKBB00186-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00186-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 54.7 428 738 53.8 387 729 St. Dev. 3.23 21.83 31.73 4.79 25.93 45.2 %CV 5.90 5.10 4.29 8.90 6.70 6.20 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 54.7 428 738 53.8 387 729 Mean (pg/mL) 54.7 428 738 53.8 387 729 St. Dev. 3.23 21.83 31.73 4.79 25.93 45.2 St. Dev. 3.23 21.83 31.73 4.79 25.93 45.2 %CV 5.90 5.10 4.29 8.90 6.70 6.20 %CV 5.90 5.10 4.29 8.90 6.70 6.20
CAS Number
7732-18-5
Gene Name
Interleukin 4
Gene Aliases
B cell growth factor 1, B cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin, BSF 1, BSF1, IL 4, Interleukin 4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
Gene ID
287287
Accession Number
NP_958427.1
Reactivity
Rat
Immunogen
Expression system for standard: E.coli; Immunogen sequence: C25-S147
Target
Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's rat IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-4 has been precoated onto 96-well plates. Standards (E.coli, C25-S147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat IL-4 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates and serum
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant rat IL-4
NCBI Gene Symbol
IL4
Protein Name
Interleukin-4
Gene Name URL
IL4
Nucleotide Accession Number
NM_201270.1

Related Products

CatalogName
OKBB00184-96WIL-4 ELISA Kit (Human)
OKBB00181-96WIL-2 ELISA Kit (Rat)
OKBB00191-96WIL-6 ELISA Kit (Rat)
OKBB00390-96WIL-3 ELISA Kit (Rat)
OKBB00358-96WIL-13 ELISA Kit (Rat)
OKCD01459IL-4 ELISA Kit (Chicken) (OKCD01459)
OKBB00185IL-4 ELISA Kit (Mouse) (OKBB00185)
OKCD05846-96WIL-4 ELISA Kit (Guinea Pig)
OKBB00184IL-4 ELISA Kit (Human) (OKBB00184)
OKCD07534IL-10RA ELISA Kit (Rat) (OKCD07534)

Alternative Products

CatalogName
GTR18851627Rat IL-4 ELISA KIT
GTR18820006-01Rat IL-4 ELISA Set
E16RE0004-048Rat IL-4 ELISA KTI
MBS495330-01IL-4 (Rat) ELISA Kit
KET9003-01ELISA kit for Rat IL-4
E2207IL-4) Rat ELISA Kit

Popular Products