Eotaxin ELISA Kit (Mouse)

CAT: 0247-OKBB00139-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00139-96WSize:96 Well With Removable Strips
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 81 201 475 98 189 458 St. Dev. 5.59 9.05 33.73 8.7 13.99 38.01 %CV 6.90 4.50 7.10 8.87 7.40 8.29 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 81 201 475 98 189 458 Mean (pg/mL) 81 201 475 98 189 458 St. Dev. 5.59 9.05 33.73 8.7 13.99 38.01 St. Dev. 5.59 9.05 33.73 8.7 13.99 38.01 %CV 6.90 4.50 7.10 8.87 7.40 8.29 %CV 6.90 4.50 7.10 8.87 7.40 8.29
CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 11
Gene Aliases
C-C motif chemokine 11, CCL 11, CCL11, CCL11_HUMAN, Chemokine (C-C motif) ligand 11, Chemokine C C motif ligand 11, Chemokine CC Motif Ligand 11, Chemokine ligand 11, Eosinophil Chemotactic P, Eosinophil chemotactic protein, Eotaxin, Eotaxin 1, MGC22554, SCYA 11, SCYA11, Small inducible cytokine A11, Small inducible cytokine subfamily A (Cys Cys) member 11, Small inducible cytokine subfamily A member 11, Small-inducible cytokine A11
Gene ID
20292
Accession Number
NP_035460.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: H24-P97
Target
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes.1 Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.2 The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse Eotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Eotaxin has been precoated onto 96-well plates. Standards (E.coli, H24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Eotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Eotaxin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (citrate, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse Eotaxin
NCBI Gene Symbol
CCL11
Protein Name
Eotaxin
Gene Name URL
CCL11
Nucleotide Accession Number
NM_011330.3

Related Products

CatalogName
OKBB00408-96WCCL24/Eotaxin-2 ELISA Kit (Mouse)
OKBB00138-96WEotaxin ELISA Kit (Human)
OKRC01146-96WOKRC01146-96W - Eotaxin-2 ELISA Kit (Mouse)
OKBB00408CCL24/Eotaxin-2 ELISA Kit (Mouse) (OKBB00408)
OKRC01145-96WOKRC01145-96W - Eotaxin-1 ELISA Kit (Mouse)
OKBB00138Eotaxin ELISA Kit (Human) (OKBB00138)
OKRC00395-96WOKRC00395-96W - Eotaxin ELISA Kit (Human)
OKRC00397-96WOKRC00397-96W - Eotaxin 3 ELISA Kit (Human)
OKAG00014Eotaxin ELISA Kit (Human) : 96 Wells (OKAG00014)
OKRC01403-96WOKRC01403-96W - Eotaxin-1 ELISA Kit (Rat)

Alternative Products

CatalogName
MBS108961-01Mouse Eotaxin 3 (EOTAXIN-3) ELISA Kit
YP-W20621-01Mouse Eotaxin 1 (Eotaxin 1) ELISA Kit
GWB-ZZD056Mouse Eotaxin ELISA Kit
NSL1570mMouse Eotaxin ELISA Kit
MBS355363-01Eotaxin Mouse ELISA Kit
EIA05616mMouse Eotaxin ELISA Kit
MBS495269-01Eotaxin (Mouse) ELISA Kit
GA-E0263MS-01Mouse Eotaxin 2 (Eotaxin 2/CCL24) ELISA Kit
GA-E0026MS-01Mouse Eotaxin 1 (Eotaxin 1/CCL11) ELISA Kit
E-UNEL-M0142-01Uncoated Mouse Eotaxin 1 (Eotaxin 1) ELISA Kit

Popular Products