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EGFR ELISA Kit (Human)

CAT: 0247-OKBB00137-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00137-96WSize:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (ng/mL) 1.37 4.68 6.21 1.49 4.92 7.14 St. Dev. 0.053 0.211 0.329 0.073 0.32 0.614 %CV 3.86 4.50 5.29 4.89 6.50 8.59 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (ng/mL) 1.37 4.68 6.21 1.49 4.92 7.14 Mean (ng/mL) 1.37 4.68 6.21 1.49 4.92 7.14 St. Dev. 0.053 0.211 0.329 0.073 0.32 0.614 St. Dev. 0.053 0.211 0.329 0.073 0.32 0.614 %CV 3.86 4.50 5.29 4.89 6.50 8.59 %CV 3.86 4.50 5.29 4.89 6.50 8.59
CAS Number
7732-18-5
Gene Name
Epidermal growth factor receptor
Gene Aliases
9030024J15Rik, AI552599, Avian erythroblastic leukemia viral (v erb b) oncogene homolog, Avian erythroblastic leukemia viral (verbb) oncogene homolog, Cell growth inhibiting protein 40, Cell proliferation inducing protein 61, EGF R, EGFR, EGFR_HUMAN, Epidermal growth factor receptor, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) Erbb, erbb 1, Erbb1, Erythroblastic leukemia viral (v erb-b) oncogene homolog, HER1, mENA, Oncogene ERBB, OTTMUSP00000005385, OTTMUSP00000005386, PIG61, Proto oncogene c ErbB 1, Proto-oncogene c-ErbB-1, Receptor tyrosine protein kinase ErbB 1, Receptor tyrosine protein kinase ErbB1, Receptor tyrosine-protein kinase ErbB-1, RP23-295E4.1, Urogastrone, wa2, Wa5
Gene ID
1956
Accession Number
NP_005219.2
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-S645
Target
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4) . EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα) . EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development.2 Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.3
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human EGFR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGFR has been precoated onto 96-well plates. Standards (NS0, M1-S645) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGFR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human EGFR amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum, plasma (heparin, EDTA) and human milk
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human EGFR
NCBI Gene Symbol
EGFR
Protein Name
Epidermal growth factor receptor
Gene Name URL
EGFR
Nucleotide Accession Number
NM_005228.3

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