T3 ELISA Kit

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Reproducibility

Sample Triiodothyronine Conc. (pg/mL) %CV 1 629.5 6.6 2 1,169.3 5.5 3 541.6 6.7 Sample Triiodothyronine Conc. (pg/mL) %CV Sample Triiodothyronine Conc. (pg/mL) %CV 1 629.5 6.6 1 629.5 6.6 2 1,169.3 5.5 2 1,169.3 5.5 3 541.6 6.7 3 541.6 6.7 Sample Triiodothyronine Conc. (pg/mL) %CV 1 682.4 11.6 2 1,193.8 14.0 3 587.7 14.7 Sample Triiodothyronine Conc. (pg/mL) %CV Sample Triiodothyronine Conc. (pg/mL) %CV 1 682.4 11.6 1 682.4 11.6 2 1,193.8 14.0 2 1,193.8 14.0 3 587.7 14.7 3 587.7 14.7

CAS Number

7732-18-5

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

The DetectXÂ® Triiodothyronine (T3) Enzyme Immunoassay kit is designed to quantitatively measure T3 present in extracted serum and plasma, urine, extracted dried fecal samples, and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples. A T3 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the T3 in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, EDTA and Heparin Plasma, Urine, TCM and Fecal Extracts

Sensitivity

37.4 pg/mL

Reconstitution

-20°C

Components

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Triiodothyronine (T3) Standard 70 uL or 350 uL DetectXÂ® Triiodothyronine (T3) Antibody 3 mL or 13 mL DetectXÂ® Triiodothyronine (T3) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Triiodothyronine (T3) Standard 70 uL or 350 uL Triiodothyronine (T3) Standard 70 uL or 350 uL DetectXÂ® Triiodothyronine (T3) Antibody 3 mL or 13 mL DetectXÂ® Triiodothyronine (T3) Antibody 3 mL or 13 mL DetectXÂ® Triiodothyronine (T3) Conjugate 3 mL or 13 mL DetectXÂ® Triiodothyronine (T3) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each

NCBI Gene Symbol

Gene Name URL

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number	7732-18-5
PubChem CID	962
IUPAC Name	oxidane
Molecular Formula	H2O
Molecular Weight	18.015
XLogP	-0.5
Topological Polar Surface Area	1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Heavy Atom Count	1
Formal Charge	0
Complexity	0

SMILES

InChI

InChI=1S/H2O/h1H2

InChIKey

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Chemical Structure

2D Structure

CAS: 7732-18-5

CID: 962

Formula: H2O

MW: 18.015

Interactive 3D Structure

Loading 3D structure...

View on PubChem Raw 3D data

Computed Properties

Property	Value
Molecular Weight	18.015
XLogP3	-0.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Exact Mass	18.010564683
Monoisotopic Mass	18.010564683
Topological Polar Surface Area	1 Å²
Heavy Atom Count	1
Formal Charge	0
Complexity	0
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes

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T3 ELISA Kit

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