ST2 ELISA Kit (Human)

Sample ST2 Conc. (pg/mL) %CV 1 1,648.7 2.2 2 1,136.5 2.6 3 585.3 8.2 Sample ST2 Conc. (pg/mL) %CV Sample ST2 Conc. (pg/mL) %CV 1 1,648.7 2.2 1 1,648.7 2.2 2 1,136.5 2.6 2 1,136.5 2.6 3 585.3 8.2 3 585.3 8.2 Sample ST2 Conc. (pg/mL) %CV 1 1,463.7 7.5 2 990.0 8.1 3 489.3 9.8 Sample ST2 Conc. (pg/mL) %CV Sample ST2 Conc. (pg/mL) %CV 1 1,463.7 7.5 1 1,463.7 7.5 2 990.0 8.1 2 990.0 8.1 3 489.3 9.8 3 489.3 9.8

7732-18-5

Human

ELISA Kit

Enzyme-linked immunosorbent assay

The DetectX® human ST2 EIA kit is designed to quantitatively measure ST2 present in a variety samples and tissue culture media. Please read the complete kit insert before performing this assay. A recombinant human ST2 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture ST2 present in the sample. After a 60 minute incubation, the plate is washed. A biotinylated ST2 antibody is added and the plate incubated for an additional 60 minutes. Following a second wash, peroxidase conjugated streptavidin is added and the plate is incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound peroxidase conjugated streptavidin. After an incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the ST2 in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, EDTA Plasma and Tissue Culture Media

30.5 pg/mL

2°C to 8°C

Component Quantity Clear Coated 96 Well Plate 1 Plate Human ST2 Standard 2 Each DetectX® ST2 Detection Antibody 5 mL Streptavidin-Peroxidase Conjugate 5 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL Stop Solution 5 mL Plate Sealer 3 each Component Quantity Component Quantity Clear Coated 96 Well Plate 1 Plate Clear Coated 96 Well Plate 1 Plate Human ST2 Standard 2 Each Human ST2 Standard 2 Each DetectX® ST2 Detection Antibody 5 mL DetectX® ST2 Detection Antibody 5 mL Streptavidin-Peroxidase Conjugate 5 mL Streptavidin-Peroxidase Conjugate 5 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL TMB Substrate 11 mL Stop Solution 5 mL Stop Solution 5 mL Plate Sealer 3 each Plate Sealer 3 each

ST2