T4 ELISA Kit

• Reproducibility:

  Sample Thyroxine Conc. (ng/mL) %CV 1 5.5 3.0 2 3.3 2.9 Sample Thyroxine Conc. (ng/mL) %CV Sample Thyroxine Conc. (ng/mL) %CV 1 5.5 3.0 1 5.5 3.0 2 3.3 2.9 2 3.3 2.9 Sample Thyroxine Conc. (ng/mL) %CV 1 5.4 6.3 2 3.3 7.8 Sample Thyroxine Conc. (ng/mL) %CV Sample Thyroxine Conc. (ng/mL) %CV 1 5.4 6.3 1 5.4 6.3 2 3.3 7.8 2 3.3 7.8

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked immunosorbent assay

• Assay Principle:

  The DetectX® Thyroxine (T4) Multi-Format Immunoassay kit is designed to quantitatively measure T4 present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total T4 in serum and plasma and in extracted fecal samples. The kit offers 2 standard curve ranges. For serum and plasma samples we recommend using 10 uL of standards or samples. The assay concentration range for T4 will be from 20 ng/mL to 0.625 ng/mL. For urine samples we recommend alternatively using 100 uL of standards or samples. Assay concentrations of T4 that range from 2 ng/mL to 0.0625 ng/mL can be measured. A T4 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A T4 -peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to T4 to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T4 -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the T4 in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, EDTA and Heparin Plasma, Urine, TCM and Fecal Extracts

• Sensitivity:

  Sensitivity was determined as 0.23 ng/mL for 10 uL and 55.3 pg/mL for 100 uL sample size.

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Thyroxine (T4) Standard 40 uL or 200 uL DetectX® Thyroxine (T4) Antibody 3 mL or 13 mL DetectX® Thyroxine (T4) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Thyroxine (T4) Standard 40 uL or 200 uL Thyroxine (T4) Standard 40 uL or 200 uL DetectX® Thyroxine (T4) Antibody 3 mL or 13 mL DetectX® Thyroxine (T4) Antibody 3 mL or 13 mL DetectX® Thyroxine (T4) Conjugate 3 mL or 13 mL DetectX® Thyroxine (T4) Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each

• NCBI Gene Symbol:

  T4

• Gene Name URL:

  T4

• CAS Number:

  7732-18-5