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Cystatin-C ELISA Kit

CAT: 0247-OKAU00011-1PLATESize: 1 PlateDry Ice: NoHazardous: No
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CAT#:0247-OKAU00011-1PLATESize:1 Plate
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Reproducibility
Sample Cystatin C Conc. (ng/mL) %CV 1 7.72 9.1 2 5.08 10.3 3 4.42 7.6 4 0.88 10.3 Sample Cystatin C Conc. (ng/mL) %CV Sample Cystatin C Conc. (ng/mL) %CV 1 7.72 9.1 1 7.72 9.1 2 5.08 10.3 2 5.08 10.3 3 4.42 7.6 3 4.42 7.6 4 0.88 10.3 4 0.88 10.3 Sample Cystatin C Conc. (ng/mL) %CV 1 7.79 8.4 2 5.48 10.2 3 4.97 11.1 4 1.04 12.4 Sample Cystatin C Conc. (ng/mL) %CV Sample Cystatin C Conc. (ng/mL) %CV 1 7.79 8.4 1 7.79 8.4 2 5.48 10.2 2 5.48 10.2 3 4.97 11.1 3 4.97 11.1 4 1.04 12.4 4 1.04 12.4
CAS Number
7732-18-5
Gene Name
Cystatin-C
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
The DetectX® Human Cystatin C kit is designed to quantitatively measure human Cystatin C present in biological samples and tissue culture media. Please read the complete kit insert before performing this assay. A human Cystatin C standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture the Cystatin C present. After a 60 minute incubation, the plate is washed and a peroxidase conjugated Cystatin C monoclonal antibody is added. The plate is again incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound Cystatin C Antibody Conjugate. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the Cystatin C in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, EDTA and Heparin Plasma, Urine and Tissue Culture Media
Sensitivity
Sensitivity was determined as 0.058 ng/mL. This is equivalent to less than 3 pg/well.
Reconstitution
2°C to 8°C
Components
Component Quantity Clear Coated 96 Well Plate One Plate Cystatin C Standard 60 uL DetectX® Cystatin C Conjugate 5 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL Stop Solution 5 mL Plate Sealer 1 each Component Quantity Component Quantity Clear Coated 96 Well Plate One Plate Clear Coated 96 Well Plate One Plate Cystatin C Standard 60 uL Cystatin C Standard 60 uL DetectX® Cystatin C Conjugate 5 mL DetectX® Cystatin C Conjugate 5 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL TMB Substrate 11 mL Stop Solution 5 mL Stop Solution 5 mL Plate Sealer 1 each Plate Sealer 1 each

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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