Recombinant Human CDK2 Protein (E.coli, His Tag)

Recombinant Human CDK2 Protein (E.coli, His Tag)

• Background: Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.
• Abbreviation: CDK2
• Synonyms: CDK2, CDKN2, Cell Division Protein Kinase 2, Cyclin-Dependent Kinase 2, p33 Protein Kinase
• CAS Number: 9000-83-3
• UniProt: P24941
• Accession Number: P24941
• Host: E. coli
• Origin Species: Human
• Tag: N-His
• Sequence: Met 1-Leu298
• Field of Research: Signal Transduction;Cell biology;Neuroscience;Cancer;epigenetics and nuclear signal;metabolism;
• Purity: > 90% as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
• Molecular Weight: 34 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.