MAT2B Protein Lysate

• Gene Name:

  Methionine adenosyltransferase 2B

• Gene Aliases:

  Beta regulatory subunit of methionine adenosyltransferase; dTDP-4-keto-6-deoxy-D-glucose 4-reductase; MAT II beta; MAT-II; MATIIbeta; methionine adenosyltransferase 2 subunit beta; Methionine adenosyltransferase II beta; methionine adenosyltransferase II, beta; Nbla02999; putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase; putative protein product of Nbla02999; SDR23E1; short chain dehydrogenase/reductase family 23E, member 1; testicular tissue protein Li 118; TGR.

• Gene ID:

  27430

• Accession Number:

  https://www.ncbi.nlm.nih.gov/protein/NP_037415

• Reactivity:

  Homo sapiens

• Target:

  Homo sapiens methionine adenosyltransferase 2B (MAT2B), transcript variant 1, mRNA, Methionine adenosyltransferase 2 subunit beta, methionine adenosyltransferase 2 subunit beta isoform 1 [Homo sapiens]

• Type:

  Protein Lysate

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Lyophilized

• Reconstitution:

  The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes

• Molecular Weight:

  37.4 kDa

• NCBI Gene Symbol:

  MAT2B

• Host or Source:

  HEK293T

• Protein Name:

  A0A140VJP2, NM_013283, NP_037415, Q9NZL9

• Preparation:

  HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . To facilitate transportation and protein, the products are supplied as lyophilized proteins.

• Gene Name URL:

  MAT2B

• Nucleotide Accession Number:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013283