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EIF4A1 Protein Lysate

CAT: 0247-OCOA00485-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0247-OCOA00485-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Name
Eukaryotic translation initiation factor 4A1
Gene Aliases
ATP-dependent RNA helicase eIF4A-1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I; eukaryotic initiation factor 4AI; eukaryotic initiation factor 4A-I; eukaryotic translation initiation factor 4A.
Gene ID
1973
Accession Number
https://www.ncbi.nlm.nih.gov/protein/NP_001407
Reactivity
Homo sapiens
Target
Eukaryotic initiation factor 4A-I, eukaryotic initiation factor 4A-I isoform 1 [Homo sapiens], Homo sapiens eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1, mRNA
Type
Protein Lysate
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Lyophilized
Reconstitution
The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Molecular Weight
46.2 kDa
NCBI Gene Symbol
EIF4A1
Host or Source
HEK293T
Protein Name
NM_001416, NP_001407, P60842
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.) . To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Gene Name URL
EIF4A1
Nucleotide Accession Number
https://www.ncbi.nlm.nih.gov/nuccore/NM_001416

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