MLKL Antibody

• Gene Name:

  Mixed lineage kinase domain-like

• Gene Aliases:

  HMLKL

• Gene ID:

  197259

• Accession Number:

  NP_001135969.1

• Reactivity:

  Human

• Immunogen:

  Synthetic Peptide of MLKL

• Target:

  This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF) -induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

• Clonality:

  Monoclonal

• Type:

  Monoclonal Antibody

• Applications:

  IHC

• Purification:

  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Reconstitution:

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Fragment:

  IgG1

• Specificity:

  MLKL Mouse Monoclonal Antibody (8H7) detects endogenous levels of MLKL

• NCBI Gene Symbol:

  MLKL

• Host or Source:

  Mouse

• Protein Name:

  Mixed lineage kinase domain-like protein

• Gene Name URL:

  MLKL

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001142497.2