CDKN1A Antibody

• Gene Name :

  Cyclin-dependent kinase inhibitor 1A (p21, Cip1)

• Gene Aliases :

  P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1

• Gene ID :

  1026; 12575

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthesized peptide derived from human p21 around the non-phosphorylation site of T145. Location: 80-160aa.

• Target :

  P21 is a protein encoded by the CDKN1A gene which is approximately 18,1 kDa. p21 is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, RET signalling, cyclins and cell cycle regulation. It may be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. It binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. It also functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. Mutations in the CDKN1A gene may result in pancreatic cancer and ovarian cancer.

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  WB

• Purification :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Format :

  Liquid.// PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Reconstitution :

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Molecular Weight :

  21 kDa

• Notes :

  Formerly GWB-GTHOMS

• Fragment :

  IgG

• Specificity :

  P21 Polyclonal Antibody detects endogenous levels of p21 protein.

• NCBI Gene Symbol :

  CDKN1A

• Host or Source :

  Rabbit

• Gene Name URL :

  CDKN1A

• CAS Number :

  9007-83-4