LAT Antibody (Phospho-Tyr191)

• Gene Name:

  Linker for activation of T cells

• Gene Aliases:

  LAT1, pp36, IMD52

• Gene ID:

  27040; 16797; 81511

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human LAT around the phosphorylation site of Y191. Location: 160-240aa.

• Target:

  The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Reconstitution:

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Molecular Weight:

  28 kDa

• Fragment:

  IgG

• Specificity:

  Phospho-LAT (Tyr191) Polyclonal Antibody detects endogenous levels of LAT protein only when phosphorylated at Y191.

• NCBI Gene Symbol:

  LAT

• Host or Source:

  Rabbit

• Gene Name URL:

  LAT

• CAS Number:

  9007-83-4