EIF4EBP1 Antibody

• Gene Name:

  Eukaryotic translation initiation factor 4E binding protein 1

• Gene Aliases:

  BP-1, 4EBP1, 4E-BP1, PHAS-I

• Gene ID:

  1978; 13685; 116636

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human 4E-BP1 around the non-phosphorylation site of T46. Location: 30-110aa.

• Target:

  This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Reconstitution:

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Molecular Weight:

  18 kDa

• Fragment:

  IgG

• Specificity:

  4E-BP1 Polyclonal Antibody detects endogenous levels of 4E-BP1 protein.

• NCBI Gene Symbol:

  EIF4EBP1

• Host or Source:

  Rabbit

• Gene Name URL:

  EIF4EBP1

• CAS Number:

  9007-83-4