ENaC Beta Antibody

• Gene Name:

  Sodium channel, non-voltage-gated 1, beta subunit

• Gene Aliases:

  SCNN1B, Amiloride sensitive sodium channel subunit beta, Amiloride-sensitive sodium channel beta-subunit, Beta ENaC, Beta NaCH, ENaC beta, ENaCB, Epithelial Na (+) channel subunit beta, Epithelial Na+ channel beta subunit, Epithelial Na+ channel subunit beta, Epithelial sodium channel beta 2 subunit, Epithelial sodium channel beta 3 subunit, Nonvoltage gated sodium channel 1 beta subunit, Nonvoltage gated sodium channel 1 subunit beta, Nonvoltage-gated sodium channel 1 beta subunit, SCNEB, SCNN 1B, Sodium channel nonvoltage gated 1 beta (Liddle syndrome), Sodium channel nonvoltage gated 1 beta

• Gene ID:

  24767

• Accession Number:

  NP_036780

• Reactivity:

  Human, Mouse, Rat, Xenopus, Hamster

• Immunogen:

  Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)

• Target:

  He Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport. Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption. ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate. The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia. And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC, ICC, IF, IP

• Purification:

  Protein A Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:100) ; optimal dilutions for assays should be determined by the user.

• Format:

  Liquid// PBS with 0.09% sodium azide and 50% glycerol

• Buffer:

  PBS, 50% glycerol, 0.09% sodium azide

• Reconstitution:

  Store at -20C. Shipping with Blue Ice or 4C.

• Notes:

  Formerly GWB-0X0038

• Specificity:

  Detects ~87kDa.

• NCBI Gene Symbol:

  ENaC

• Host or Source:

  Rabbit

• Gene Name URL:

  ENaC

• CAS Number:

  9007-83-4