Hsp70 Antibody

9007-83-4

Heat shock 70kDa protein 1A

HSP70 1, HSP70 2, HSP70.1, HSP72, HSP73, HSPA1, HSPA1A, HSPA1B

3303

NP_005336.3

Human, Mouse, Rat, Beluga, Bovine, Dog, Fish, Carp, Guinea Pig, Hamster, Monkey, Pig, Sheep, Coral, Plant, Shark, L. amazonensis

Recombinant Full length HSP70 Protein

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s) . In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity. The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins. All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Unconjugated

Polyclonal Antibody

WB, IHC, ICC, IF, IP, ELISA

Peptide Affinity Purified

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

1 mg/ml

WB (1:1000), IHC (1:100), ICC/IF (1:100), IP (1:100) ; optimal dilutions for assays should be determined by the user.

Liquid// PBS with 50% glycerol and 0.09% sodium azide

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Store at -20C. Shipping with Blue Ice or 4C.

Formerly GWB-8B4B3C

Detects a ~70kDa. May cross-react with HSC70 at lower dilutions.

HSP70

Rabbit