KCC2 Antibody

• Gene Name:

  Solute carrier family 12 (potassium-chloride transporter), member 5

• Gene Aliases:

  Potassium Chloride Cotransporter, Potassium chloride transporter 5, SLC12A5, hKCC2, S12A5, Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter, KIAA1176, Electroneutral potassium chloride cotransporter 2, Erythroid K Cl cotransporter 2, Furosemide sensitive K Cl cotransporter, K-Cl cotransporter 2, KCC 2, Neuronal K Cl cotransporter, Solute carrier family 12 (potassium chloride transporter) member 5, Solute carrier family 12 member 5

• Gene ID:

  171373

• Accession Number:

  NP_599190

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Fusion protein amino acids 932-1043 corresponding to rat KCC2

• Target:

  KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.

• Clonality:

  Monoclonal

• Clone:

  S1-12

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Applications:

  WB, IHC, ICC, IF, IP

• Purification:

  Protein G Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:300), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Format:

  Liquid.// PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide.

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Reconstitution:

  Store at -20C. Shipping with Blue Ice or 4C.

• Molecular Weight:

  140 kDa

• Fragment:

  IgG2a

• Specificity:

  Detects ~140kDa.

• NCBI Gene Symbol:

  KCC2

• Host or Source:

  Mouse

• Gene Name URL:

  KCC2

• CAS Number:

  9007-83-4