Mouse TCRb Antibody

9007-83-4

T-complex protein 1 subunit beta

Ti, Tib, TCRbe, TCRbeta

21577

Mouse

TCR affinity purified from mouse T-cell hybridoma DO-11.10

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC) . Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes several trypsinogen genes.

H57-597

Unconjugated

Polyclonal Antibody

FC, IHC, ICC, IP, STIM

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

0.5 mg/mL

6 cells Biotin conjugate 6 cells R-phycoerythrin conjugate 6 cells Allophycocyanin conjugate 6 cells Spectral Red conjugate 6 cells R-phycoerythrin-Cyanine 5.5 conjugate AF488 and AF647 6 cells 6 cells6 cells R-phycoerythrin conjugate 6 cells Allophycocyanin conjugate 6 cells Spectral Red conjugate 6 cells R-phycoerythrin-Cyanine 5.5 conjugate AF488 and AF647 6 cells 6 cells6 cells Allophycocyanin conjugate 6 cells Spectral Red conjugate 6 cells R-phycoerythrin-Cyanine 5.5 conjugate AF488 and AF647 6 cells 6 cells6 cells Spectral Red conjugate 6 cells R-phycoerythrin-Cyanine 5.5 conjugate AF488 and AF647 6 cells 6 cells6 cells R-phycoerythrin-Cyanine 5.5 conjugate AF488 and AF647 6 cells 6 cells6 cells 6 cells6 cells

Store at 2-8C

Formerly GWB-8B42B6

IgG

TCRb

TCRB

Hamster