Mouse Lambda Antibody : TRITC

• Gene Name:

  Immunoglobulin lambda-like polypeptide 1

• Gene Aliases:

  Igl-x

• Gene ID:

  111519

• Immunogen:

  Mouse lambda light chains

• Target:

  Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. This locus has four J-C clusters and three V segments that constitute two transcriptional units. One of the J-C clusters is incapable of encoding a protein; the J and C gene segments in this cluster are considered pseudogenes. Inbred strains of laboratory mice lack an additional J-C cluster of pseudogenes and several additional V segments that are found in wild mice.

• Clonality:

  Polyclonal

• Conjugation:

  TRITC: Tetramethylrhodamine

• Type:

  Secondary Antibody

• Applications:

  FLISA, IHC-F, IHC-P, ICC

• Purification:

  Affinity chromatography on pooled mouse Igs with λ light chains covalently linkedto agarose.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1.0 mg/mL

• Dilution:

  6 cells 6 cells 6 cells6 cells 6 cells6 cells

• Reconstitution:

  Store at 2-8C

• Fragment:

  IgG

• Specificity:

  Lambda

• NCBI Gene Symbol:

  IGL

• Host or Source:

  Goat

• Gene Name URL:

  IGL

• CAS Number:

  9007-83-4