Human IgM Antibody : AP

• Gene Name:

  Ig mu chain C region

• Gene Aliases:

  MU, VH, AGM1

• Gene ID:

  3507

• Reactivity:

  Human

• Immunogen:

  Human IgM myeloma protein

• Target:

  Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910) . The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005) .[supplied by OMIM, Aug 2010]

• Clonality:

  Monoclonal

• Clone:

  SA-DA4

• Conjugation:

  AP

• Type:

  Monoclonal Antibody

• Applications:

  IHC-F, ICC, WB

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  Lot specific

• Dilution:

  6 cells 6 cells 6 cells 6 cells 6 cells 1:500-1:1,000 1:1,000-1:2,000 1:1,000-1:2,0006 cells 6 cells 6 cells 6 cells 1:500-1:1,000 1:1,000-1:2,000 1:1,000-1:2,0006 cells 6 cells 6 cells 1:500-1:1,000 1:1,000-1:2,000 1:1,000-1:2,0006 cells 6 cells 1:500-1:1,000 1:1,000-1:2,000 1:1,000-1:2,0006 cells 1:500-1:1,000 1:1,000-1:2,000 1:1,000-1:2,000

• Reconstitution:

  Store at 2-8C

• Notes:

  Formerly GWB-B2E4E7

• Fragment:

  IgG1

• Specificity:

  IgM

• NCBI Gene Symbol:

  IGHM

• Host or Source:

  Mouse

• Gene Name URL:

  IGHM

• CAS Number:

  9007-83-4