Anti-GFP Antibody

• Gene Aliases:

  Green Fluorescent Protein Antibody

• Reactivity:

  Green Fluorescent Protein

• Immunogen:

  Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.

• Target:

  Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca (2+) -activated photoprotein aequorin.

• Clonality:

  Monoclonal

• Clone:

  9F9.F9

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Applications:

  Chromatin immunoprecipitation|Dot blot|Enzyme-linked immunosorbent assay|Flow cytometry|Immunofluorescence|Immunohistochemistry|Immunoprecipitation|Multiplex assay|Western blot

• Purification:

  GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (Catalog # OARA01928) from Aequorea victoria by both Western blot and ELISA.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Homology:

  Wt, rGFP, eGFP

• Format:

  Liquid (sterile filtered)

• Reconstitution:

  Anti-GFP Antibody at -20 C prior to opening. Aliquot Anti-GFP and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Notes:

  Formerly GWB-D2720A

• Specificity:

  No reaction is seen against RFP.

• Host or Source:

  Mouse

• Protein Name:

  Green fluorescent protein

• CAS Number:

  9007-83-4