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GFP Antibody

CAT: 0247-OARA01900-25ULSize: 25 µLDry Ice: NoHazardous: No
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CAT#:0247-OARA01900-25ULSize:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Green fluorescent protein
Gene Aliases
Chicken anti-GFP antibody, chicken Anti-Green Fluorescent Protein Antibody, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish anibody
Reactivity
Green Fluorescent Protein
Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Target
Chicken Anti-GFP is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. GFP Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower backgrounds for western blotting, ELISA and Immunohistochemistry.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Polyclonal Antibody
Applications
Dot blot|Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Multiplex assay|Purification|Western blot
Purification
Anti-GFP Antibody IgY purification was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1.17 mg/ml
Format
Liquid (sterile filtered)
Reconstitution
Store Anti-GFP Antibody at -20C or below prior to opening. This vial contains a relatively low volume of reagent (25 μ L) . To minimize loss of volume dilute 1:10 by adding 225 μ L of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is three (3) months from date of opening.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) . No reaction was observed against Human, Mouse or Rat serum proteins.
NCBI Gene Symbol
GFP
Host or Source
Chicken
Protein Name
Green fluorescent protein
Gene Name URL
GFP