CHAT Antibody

• Gene Name:

  Choline O-acetyltransferase

• Gene Aliases:

  CMS6, CMS1A, CMS1A2, CHOACTASE

• Gene ID:

  1103

• Reactivity:

  Human, Mouse, Chicken, Guinea Pig

• Immunogen:

  Native choline acetyltransferase purifed from human placenta

• Target:

  Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999) . Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993) . It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC

• Purification:

  Affinity Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  100 ul in in PBS + 5 mg/ml BSA + 0.2% NaN3.

• Reconstitution:

  For long term storage -20C is recommended. Stable at -20C for at least 1 year. Avoid freeze/thaw cycles.

• Molecular Weight:

  70 kDa

• Specificity:

  Specific for ~70k ChAT protein.

• NCBI Gene Symbol:

  CHAT

• Host or Source:

  Goat

• Gene Name URL:

  CHAT

• CAS Number:

  9007-83-4