Anti-GAP-43

• Gene Aliases:

  Basp2

• Gene ID:

  29423

• Accession Number:

  NP_058891.1

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide corresponding to amino acid residues from the C-terminal region of the rat GAP43 sequence.

• Target:

  GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997) . There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995) . GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, IF

• Purification:

  Total IgY fraction.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  15 mg/ml

• Format:

  Liquid.// Total IgY fraction in PBS + 10mM NaN3. Adequate amount of material to conduct 10-mini Western blots3

• Reconstitution:

  For long term storage -20C is recommended. Stable at -20C for at least 1 year.

• Molecular Weight:

  ~43kDa

• Specificity:

  Specific for the ~43k GAP-43 protein. The antibody has been directly tested for reactivity in Western blots with human, mouse, and rat tissue.

• NCBI Gene Symbol:

  GAP43

• Host or Source:

  Chicken

• Protein Name:

  Neuromodulin

• Gene Name URL:

  GAP43

• CAS Number:

  9007-83-4