Anti-GABA Alpha Receptor, alpha 3 -Subunit

• Gene ID:

  24947

• Reactivity:

  Mouse, Rat

• Target:

  Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004) . Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha -subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC, DB

• Purification:

  Affinity purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  Varies by lot. See vial for concentration.

• Format:

  Liquid// 10mM HEPES (pH 7.5), 150mM NaCl, 100 ug/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots.

• Reconstitution:

  For long term storage -80° C is recommended, but shorter term storage at -20° C is also acceptable as aliquots may be taken without freezing/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20° C.

• Molecular Weight:

  ~51kDa

• Specificity:

  Specific for the ~51k alpha 3-subunit of the GABAA-R receptor in Western blots of rat and mouse brain lysate.

• NCBI Gene Symbol:

  GABRA3

• Host or Source:

  Rabbit

• Protein Name:

  Gamma-aminobutyric acid receptor subunit alpha-3

• Gene Name URL:

  GABRA3

• CAS Number:

  9007-83-4