GLRA1 Antibody

CAT: 0247-OAPC00117-200UGSize: 200 µgDry Ice: NoHazardous: No
CAT#:0247-OAPC00117-200UGSize:200 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Aliases
GLYRA1
Gene ID
25674; 24397
Accession Number
NP_037265.1NP_036700.1
Reactivity
Human, Mouse, Rat
Immunogen
Peptide from the N-terminus region of the alpha 1-subunit of rat glycine receptor.
Target
Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003) . The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000) . The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997) . These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001) . The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IHC, DB
Purification
Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Homology
Human, Mouse, Rat
Format
Lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Reconstitution
Packaging: //200 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate.Adequate amount of material to conduct 10-mini Western Blots. Reconstitution: //The antibody should be reconstituted in 100 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2HPO4, pH 7.4) before use. Storage: //After reconstitution the antibody should be aliquoted and stored at -20C.Reconstitution: //The antibody should be reconstituted in 100 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2HPO4, pH 7.4) before use. Storage: //After reconstitution the antibody should be aliquoted and stored at -20C.Storage: //After reconstitution the antibody should be aliquoted and stored at -20C.
Molecular Weight
~48kDa
Notes
Formerly GWB-C33F7E
Specificity
Specific for the ~48k alpha 1- and alpha 2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts containing the recombinant human Glycine Receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the alpha1 subunit. Does not recognize other glycine receptor subunits.
NCBI Gene Symbol
GLRA1
Host or Source
Rabbit
Protein Name
Glycine receptor subunit alpha-1, Glycine receptor subunit alpha-2
Gene Name URL
GLRA1GLRA2

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