Anti-DARPP-32

• Gene Aliases :

  Darpp32, Darpp-32

• Gene ID :

  360616

• Accession Number :

  NP_612530.1

• Reactivity :

  Human, Mouse, Rat, Cow, Dog

• Immunogen :

  Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH) .

• Target :

  DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998) . The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999) . Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002) .

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  WB, IHC

• Purification :

  Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Homology :

  Cow; Dog; Human; Mouse; Rat

• Format :

  Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

• Reconstitution :

  Store at -20C; stable for at least one year.

• Molecular Weight :

  ~32kDa

• Notes :

  Formerly GWB-369615

• Specificity :

  Specific for the ~32k DARPP-32 protein.

• NCBI Gene Symbol :

  PPP1R1B

• Host or Source :

  Rabbit

• Protein Name :

  Protein phosphatase 1 regulatory subunit 1B

• Gene Name URL :

  PPP1R1B

• CAS Number :

  9007-83-4