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Anti-Vimentin

CAT: 0247-OAPC00085-100ULSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0247-OAPC00085-100ULSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene ID
7431
Accession Number
NP_003371.2
Reactivity
Human, Mouse, Rat, Cow
Immunogen
Recombinant human vimentin purified from E. coli.
Target
Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006) . Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons) . Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008) .
Clonality
Monoclonal
Type
Monoclonal Antibody
Applications
WB, IF
Purification
Affinity purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Homology
Cow; Human; Mouse; Rat
Format
Liquid. Contains 10 mM sodium azide.
Reconstitution
Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Molecular Weight
~50kDa
Specificity
Specific for the ~50kDa vimentin protein.
NCBI Gene Symbol
VIM
Host or Source
Mouse
Protein Name
Vimentin
Gene Name URL
VIM

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