VPS53 Antibody

• Gene Name:

  VPS53 subunit of GARP complex

• Gene Aliases:

  HCCS1; hepatocellular carcinoma suppressor 1; hVps53L; PCH2E; pp13624; vacuolar protein sorting-associated protein 53 homolog; VPS53, GARP complex subunit.

• Gene ID:

  55275

• Accession Number:

  https://www.ncbi.nlm.nih.gov/protein/EAW90658

• Reactivity:

  Chicken|Human|Mouse|Rat

• Immunogen:

  VPS53 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human VPS53. The immunogen is located within the last 50 amino acids of VPS53.

• Target:

  Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN) . The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:15878329, PubMed:18367545) . Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot

• Purification:

  Affinity Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Homology:

  Human; Mouse; Rat

• Format:

  Liquid. VPS53 Antibody is supplied in PBS containing 0.02% sodium azide.

• Reconstitution:

  VPS53 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• NCBI Gene Symbol:

  VPS53

• Host or Source:

  Rabbit

• Protein Name:

  Vacuolar protein sorting 53 (yeast), isoform CRA_a [Homo sapiens]|Vacuolar protein sorting-associated protein 53 homolog

• Gene Name URL:

  VPS53

• CAS Number:

  9007-83-4