RAP80 Antibody

• Gene Name:

  Ubiquitin interaction motif containing 1

• Gene Aliases:

  BRCA1-A complex subunit RAP80; RAP80; receptor-associated protein 80; retinoid X receptor-interacting protein 110; Ubiquitin interaction motif-containing protein 1; X2HRIP110.

• Gene ID:

  51720

• Accession Number:

  https://www.ncbi.nlm.nih.gov/protein/EAW85043

• Reactivity:

  Human|Mouse|Porcine

• Immunogen:

  RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RAP80. The immunogen is located within amino acids 520 - 570 of RAP80.

• Target:

  Ubiquitin-binding protein (PubMed:24627472) . Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.37) . Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) . The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot

• Purification:

  Affinity Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Homology:

  Human; Mouse

• Format:

  Liquid. RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.

• Reconstitution:

  RAP80 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• NCBI Gene Symbol:

  UIMC1

• Host or Source:

  Rabbit

• Protein Name:

  BRCA1-A complex subunit RAP80|receptor associated protein 80, isoform CRA_c [Homo sapiens]

• Gene Name URL:

  UIMC1

• CAS Number:

  9007-83-4