BCL2 Antibody

• Gene Name:

  BCL2 apoptosis regulator

• Gene Aliases:

  Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; PPP1R50; protein phosphatase 1, regulatory subunit 50.

• Gene ID:

  596

• Accession Number:

  https://www.ncbi.nlm.nih.gov/protein/AAH27258

• Reactivity:

  Bovine|Chicken|Human|Mouse|Rat

• Immunogen:

  Anti-Bcl-2 antibody (3337) was raised against a peptide corresponding to 14 amino acids near the center of human Bcl-2. The immunogen is located within amino acids 80-130 of Bcl-2.

• Target:

  Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Western blot

• Purification:

  Ion Exchange

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Homology:

  Human

• Format:

  Liquid. Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Reconstitution:

  Bcl-2 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Molecular Weight:

  Predicted: 26kD Observed: 26 kD

• Notes:

  Formerly GWB-73166A

• NCBI Gene Symbol:

  BCL2

• Host or Source:

  Rabbit

• Protein Name:

  Apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2 [Homo sapiens]

• Gene Name URL:

  BCL2

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_000633.2NM_000657.2