TRIM5 Antibody

9007-83-4

Tripartite motif containing 5

RING-type E3 ubiquitin transferase TRIM5; TRIM5alpha; tripartite motif-containing 5/cyclophilin A fusion protein; tripartite motif-containing protein 5.

574288

https://www.ncbi.nlm.nih.gov/protein/ABG67967

Human|Rhesus Monkey

TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha. The immunogen is located within the last 50 amino acids of TRIM5 alpha.

Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3) . Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV-agm) . Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 (PubMed:25127057) . Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction (PubMed:25127057) .

Unconjugated

Polyclonal Antibody

Enzyme-linked immunosorbent assay|Immunofluorescence|Western blot

Affinity Purified

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

1 mg/ml

Human

Liquid. TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide.

TRIM5 alpha antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Predicted: 54 kDa Observed: 55 kDa

Formerly GWB-22B641

Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein.

TRIM5

Rabbit

Tripartite motif-containing 5 alpha isoform [Macaca mulatta]|Tripartite motif-containing protein 5

TRIM5