BCL2 Antibody

• Gene Aliases:

  Bcl-2, PPP1R50

• Gene ID:

  596

• Accession Number:

  NP_000624.2

• Reactivity:

  Human

• Immunogen:

  The immunogen for anti-BCL2 antibody: anti-human BCL2 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human BCL2 amino acids 1-211 purified from E. coli.

• Target:

  BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

• Clonality:

  Monoclonal

• Clone:

  PAT1B5AT

• Type:

  Monoclonal Antibody

• Applications:

  IF, WB

• Purification:

  BCL2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Format:

  Liquid// PBS (pH 7.4) with 0.1% sodium azide

• Reconstitution:

  For periods up to one month, store at 4C. For periods up to 12 months, store at -20C. Prevent freeze/thaw cycles.

• Notes:

  Formerly GWB-BSP884

• Fragment:

  Mouse IgG2b heavy chain and kappa light chain.

• NCBI Gene Symbol:

  BCL2

• Host or Source:

  Mouse

• Protein Name:

  Apoptosis regulator Bcl-2

• Gene Name URL:

  BCL2

• CAS Number:

  9007-83-4